Reporter

Part:BBa_K511103:Design

Designed by: Grant Robinson   Group: iGEM11_MIT   (2011-09-28)

amCyan-miRFF4 MammoBlock


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 329
    Illegal PstI site found at 881
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 329
    Illegal PstI site found at 881
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 329
    Illegal XhoI site found at 226
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 329
    Illegal PstI site found at 881
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 329
    Illegal PstI site found at 881
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Not applicable.


Source

This protein is an engineered version of the original green fluorescent protein from Aequorea victoria that has ben modified to fluoresce cyan and includes a concatenation of the synthetic FF4 miRNA sequence. Sincere thanks go to the Weiss lab for contributing this entry vector to the MIT iGEM 2011 team.

References